This study introduces an innovative approach for noise-free and aberration-reduced reconstruction of optical coherence tomography (OCT) images of the healthy human cornea, acquired in-vivo with a high-speed, cellular-resolution Line-Field OCT. The proposed method utilizes non-local image priors within a maximum-a-posteriori (MAP) framework, followed by further reconstruction based on light backpropagation models, to address the diffraction-induced artifacts. The performance of the algorithm was tested on different images acquired with the LF-OCT system including resolution targets, plant tissues, and human cornea.

We report the effectiveness of a numerical refocusing technique for ex vivo mouse retinal imaging using a single-shot optical coherence microscopy (OCM) compared to conventional multiple OCM acquisitions with adjustable focus control. We also investigated the performance of numerical refocusing with different raster scanning patterns and proposed a novel phase bias estimation and correction method for effective OCM imaging. This method will further enhance the functionality of OCM for temporal dynamic analysis of cellular components in mouse retinal tissue.

A label-free method for tissue dynamics imaging, so-called dynamic OCT is presented. The detailed principle and in vitro and ex vivo applanations are presented. In addition, deep-learning of high-speed dynamic OCT and new quantitative dynamic OCT algorithms are presented.

The effective management of eye diseases increasingly depends on fundus photography, highlighting the need for innovative, accessible, and mobile imaging technologies to advance teleophthalmology. Conventional fundus cameras, limited by trans-pupillary illumination, have a restricted field of view and frequently necessitate drug-induced pupillary dilation for detailed eye examinations. Our research addresses these issues by introducing alternative illumination techniques, facilitating the creation of compact, affordable widefield fundus cameras. Utilizing miniaturized indirect illumination, our devices achieve widefield imaging (>100° eye-angle) without requiring pupillary dilation. Additionally, we have successfully employed trans-pars-planar and trans-palpebral illumination strategies, proving their efficacy in achieving ultra-widefield imaging (>200° eye-angle) for both pediatric and adult patients. The incorporation of low-cost imaging components and smartphone integration into our portable units not only makes multispectral fundus imaging widely accessible but also significantly enhances the reach and efficiency of teleophthalmology services, thereby improving access to comprehensive eye care.